Microbial communities in an anammox reactor treating municipal wastewater at mainstream conditions: Practical implications of different molecular approaches

Biases on molecular techniques used to study microbial communities in the anammox process are not always considered. This work aimed evaluate different approaches bacterial an reactor treating real urban wastewater at mainstream conditions. 16S rRNA amplicon sequencing with primer sets, metagenomics, and qPCR were evaluated. An enriched feed synthetic was as a positive control. We reported that, despite its silico coverage, universal primers 515F-909R had, fact, very low coverage (1%) of Planctomycetota phylum. The functional genes approach showed taxonomic resolution species level, although genomic database updates bias be From phylogenetic analysis, coherence among obtained. Primers targeting hydrazine synthase consistency quantification, making them useful for comparing similar investigations. According results, recommendations biomolecular outlined.